TOAST Amplicon Designer Tool
A novel tool for designing targeted gene amplicons and an optimised set of primers for high-throughput sequencing in tuberculosis genomic studies
TOAST is a flexible tool for designing targeted amplicon panels to detect TB drug resistance.
It uses mutation frequency data from over 50,000 genomes to prioritize high-value regions for primer selection.
The final 33-amplicon panel achieved over 97% coverage of key resistance markers and was validated using Nanopore sequencing.
TOAST is available as a command-line tool, Python package, and interactive web app with support for custom inputs.
Amplicon Design Tool Interface
Primer Coverage Distribution
